A HTML/JavaScript tool to fit and simulate circular dichroism spectra of proteins, rewritten into JavaScript from the Excel program described in this article.
Step 1: To fit an experimental spectrum, paste data in text format here
Load from file:
Sample data set:
Step 2: Click run for CD fits/simulations
(If no experimental data are given, clicking Run allows simulations of CD spectra)
Result: experimental and simulated CD spectra
Notes: Secondary structure contents from CD fits might have 10-20 % error...
This tool is based on multilinear regression to reference spectra as explained here but with javascript-only code
Simulation controls
Alpha coefficient...
Beta coefficient...
Turn coefficient...
Random coil...
(so that the coefficients become % of each secondary structure type)
Show reference spectra (red for helix, green for beta, blue for turn, yellow for random coil)